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Fluid flow and heat transfer are widely occurred in the engineering processes, which can be in the multiscale forms, i.e. nano/micro or macro. As the technologies rapidly evolving, the advanced fluids such as nanofluids and supercritical fluids are more and more widely applied in the different engineering areas. The course aims to polish the knowledge of multiscale fluid flow and heat transfer to the graduate students majoring in mechanical engineering, and encourage them to apply the principles and theories into the research and practices. The topics cover the fluid mechanics and heat transfer for different fluids types, which include: single-phase fluid, two-phase flow, supercritical fluid, and nanofluids.